Address
10 place de la Joliette
Atrium 10.4 – Etage 6
13002 Marseille, France
Name: Luca Ravotto
University / Institution: University of Zurich
Department / Research Group: Experimental Imaging and Bioenergetics – Dept. Pharmacology and Toxicology
Position / Title: Senior Scientist
Research Area / Specialization: Multiphoton Microscopy
1. Please briefly describe your research focus and current projects.
We work on fluorescent imaging in the mouse brain, to study metabolism, neurotransmission and hemodynamics. My role in particular is focused on the implementation of optical techniques (with a focus on Fluorescence Lifetime Imaging) and data analysis tools.
2. What research needs or challenge were you aiming to address when looking for a
solution ?
As part of our studies, we were implementing a mini2P microscope for brain imaging of freely behaving mice.
3. What factors influenced your decision to select our system ?
The photonics crystal fiber from LightCore offered a unique combination of low dispersion across a broad wavelength range.
4. How has your experience been using our system in your research work ?
The fiber is of high quality and worked with no problem. The custom-made collimation adapter for the mini2P was also robust and well functional. The coupling box offered an easy alignment experience.
5. Have our system contributed to any improvements in your workflow, experiments,
efficiency, or research outcomes ?
Significant simplification of alignment and flexibility in the choice of the excitation wavelength.
6. Are there any specific features or aspects you particularly value ?
The broad wavelength range in which the fiber can be operated.
7. Would you recommend our system to other researchers or institutions ? Why ?
Definitely, and in fact I already have. For the transport of femtosecond pulses for multiphoton excitation the fiber from LightCore is the only one I know of to offer high transmittance and very low dispersion across a very wide wavelength range.