10 place de la Joliette
Atrium 10.4 – Etage 6
13002 Marseille, France
The InSplorer™ endoscope is a flexible miniature probe that facilitates multimodal nonlinear imaging. This includes two-photon and three-photon fluorescence, harmonic generation (Second Harmonic Generation – SHG, Third Harmonic Generation – THG), and Coherent Anti-Stokes Raman Scattering (CARS) imaging. Its applications are diverse, encompassing biological cell or fiber imaging, in-vivo tissue imaging or cell activity, and even the imaging of inorganic materials.
Endoscopic probe distal head
The distal endoscopic head of the InSplorer™ endoscope houses a spiral scanner and optics, all contained within a metallic tube that measures 2 to 3 mm in diameter and 40 mm in length. The entire assembly weighs less than 2 grams. The endoscope’s flexible length extends from 2 to 4 meters, providing ample freedom of movement around the room. It is capable of imaging a Field of View (FoV) up to 600 µm, with a frame rate of up to 10 Hz, a point spread function (PSF) of approximately 1 µm, and a working distance of up to 600 µm. Signal delivery is facilitated by a double-clad hollow core fiber, which ensures broadband transmission of short pulses without pulse distortion.
Our InSplorer™ endoscope is connected to a standalone cabinet that encompasses all the necessary components to operate it. The distal head can be plugged into the front for scanner calibration. All electronics are consolidated with straightforward on/off buttons located on the front. The control PC, equipped with a monitor/keyboard arm attached to the side of the cabinet, offers enhanced flexibility. A laser and its control unit are integrated into the cabinet, culminating in a truly standalone system. Currently available lasers include 780 or 920 nm femtosecond sources from Toptica. A flexible solution to combine with a table-top laser, based on a fiber delivery, is also available.
The InSplorer™ endoscope is managed by an intuitive software that simplifies the imaging process. This software facilitates the calibration of the spiral scanner, including an automatic calibration algorithm for ease of use. It also provides quantitative signal measurement and imaging capabilities. Laser power management is incorporated to ensure uniform illumination across the sample. Additionally, the software includes integrated post-processing tools to enhance the comfort and efficiency of the imaging routine.